Tuesday, March 17, 2020

6 Budget-Friendly Ways to Get Writing Help for Your Paper of Any Complexity

6 Budget-Friendly Ways to Get Writing Help for Your Paper of Any Complexity 6 Budget-Friendly Ways to Get Writing Help for Your Paper of Any Complexity It’s no secret that college can be extremely expensive, and most college students are on tight budget. You may not afford writing help for a high price, but we’ve created a list of six budget-friendly options. Here are several ways to get the qualitative help you need without breaking the bank. 1.   Pay for Advisory Services Only The cheapest option for academic writing help is to pay only for general guidance on your writing, rather than for an entire paper. This service can include brainstorming a good topic, organizing, structuring your essay and proofreading/editing your completed draft. Such writing helpers can give you the extra boost you need to write an excellent paper on your own. 2. Pay an Older Student to Write Your Paper Many senior college students write papers for younger ones to make extra cash. Ask around your college campus to see if there are students available to perform such a service. While you are searching for writer help, make sure you are able to get opinion on the quality of this person’s work. To pay a senior student for the paper is a great chance to save money, as they are likely to charge much less than professional services. 3. Think about Friends Who May Do You a Favor Do you have any friends who are excellent writers? Perhaps they would be generous enough to write a paper for you, or, at least, to offer suggestions and advice. If you are strong in another academic subject, such as mathematics, you may provide your mate with â€Å"friend help† in exchange. Even if your friend wants some money for such a favor, he/she will charge you less than a professional academic writer would. 4. Use a Free Online Library There are several resources on the Internet, such as free online libraries, that provide you with papers on a variety of subjects. These papers can be used as examples to help inspire ideas for your essay. You can also use the sample essay as a model for how to structure your own paper. Such a method does not allow you to get writing help from another person, it just gives you a chance to see how a proficient writer has handled the topic similar to the one you are coping with. 5. Ask Your Professor for Help Of course, your professor is not going to write the paper in your stead, but he/she may offer you valuable advice. Most professors are required to have office hours, the time period, when the professor sits in his/her office so that students can come and ask their questions. Your professor can clarify any questions you have concerning the assignment, as well as he/she can give you tips on how to overcome the obstacles you have faced. Your professor is the one who will assign your grade, so his/her advice can be especially helpful. Moreover, professors are likely to be more generous while grading a student who has taken the initiative to come for advice! 6. Join a Study Group There are many study groups in the universities for students to get together and work on assignments, or answer one another’s questions. See if the class you are writing the paper for has a study group, or try to start one. You and students who are dealing with the papers on the same subject can discuss topic ideas, clarify questions, and even proofread one another’s papers. Getting qualitative writing help does not always put a strain on your finances. Try using one of these six options to help you produce a high-quality paper at a low cost!

Saturday, February 29, 2020

An Experiment Conducted in Attempt to Transform DNA off the Bacteria E. Coli

An Experiment Conducted in Attempt to Transform DNA off the Bacteria E. Coli Abstract: The main objective of this experiment was to transform DNA found in bacteria. A plasmid was used on E. coli and with the use of heat shock, was inherited by the bacteria which caused the E. coli to become resistant to ampicillin. Also in the plasmid was GFP which verified the hypothesis by glowing green under UV light. The predicted outcome held true and gives insight on what the future of medicine might hold. Introduction: Transforming DNA on bacteria will require a change in their genes. Using pGLO in this experiment allowed the DNA on Escherichia coli (E. coli) to transform. There are three genes in this plasmid: the araC gene, green fluorescent protein gene (GFP), and the bla gene. The ara C gene is a bifunctional regulator which transcripts araC mRNA which translates to produce araC proteins that act as a depressor or promoter to the GFP. The GFP also transcripts to produce GFP mRNA which translated to produce GFP that glows green under ultraviolet light (UV light). The last gene is the bla gene which gives the bacteria ampicillin resistance. E. Coli was used in this experiment because it is found inside our bodies so it is not very harmful to humans and grows rapidly. Bacteria transformation is the process by which foreign DNA is introduced into a cell (addgene.org). Using plasmids, A linear or circular double-stranded DNA that is capable of replicating independently of the chromosomal DNA(biolog y-online.org), transformation is able to occur. Using heat shock, most of the bacteria accepts the foreign DNA and incorporates it into its own DNA. In this experiment E. coli was transformed to become resistant to the antibiotic ampicillin and expressed the GFP. Using heat shock and pGLO will transform E. coli to be resistant to ampicillin while glowing green. These results could help transform human DNA genes and give resistance to life-threatening diseases such as AIDS. Methods and Materials: Two micro test tubes were labeled +pGLO and the other –pGLO. Using a sterile transfer pipette, 250Â µL of transformation solution (CaCl2) were transferred to each test tube. The test tubes were placed in a bucket of ice for 3 minutes. A single colony of E. coli was placed in the transformation solution (CaCl2) of the test tube labeled +pGLO with a sterile loop and spun until the colony was all the way in the transformation solution. The same was done for the test tube labeled –pGLO. After, both test tubes were placed back in the bucket of ice for another 3 minutes. A new sterile loop was used to transfer pGLO Plasmid DNA to the test tube labeled +pGLO, not –pGLO. The test tubes were then back in the ice bucket for 10 minutes. While the test tubes were in the ice, the agar plates were gathered and labeled with the plate type and group name. After 10 minutes of the test tubes being in ice, they were both transferred to a water bath set at 42Â °C for 50 seconds. After 50 seconds the test tubes were placed back in the ice bucket for 2 minutes. Taking both test tubes out, a sterile pipette was used to pipette 250Â µL of LB nutrient broth into a test tube then mixed well. The same procedure was done to the other test tube using a new sterile pipette. Once both were mixed, they were incubated at room temperature for 20 minutes. After the 20 minutes are done and using a new sterile pipette for each tube, 100Â µL of the transformation and control suspensions were transferred to the corresponding agar plates. Using a new sterile loop for each plate, the suspensions were evenly spread around the surface of the LB nutrient on each plate. The plates were stacked, labeled with a group name, and placed upside down in a fridge at 37Â °C until next week. Independent Variable: Whether or not the plate contained pGLO Dependent Variable: Growth rate Controlled Variables: Amount of LB nutrient Amount of transformation solution Amount of suspensions Time in the ice bucket and room temperature Temperature of water bath and fridge Positive Control: -pGLO, LB Negative Control: -pGLO, LB, AMP These controls were selected because even without the plasmid, the plate labeled ‘-pGLO, LB’ should have growth on it because the only thing added to the plate was nutrients for the bacteria. The plate without the plasmid, LB nutrient, and ampicillin is a negative control because the ampicillin should kill off all the bacteria on the plate. Discussion: The hypothesis was supported in this experiment. In the plate labeled ‘+pGLO, LB, AMP, ARA’ there was growth when normally the ampicillin would kill of the bacteria, as shown in the plate labeled ‘-pGLO, LB, AMP’. Also looking at the UV Light figure, the same plate was able to glow as a cause of inheriting the GFP and being in the presence of arabinose sugar. In the plate labeled ‘-pGLO, LB’ there should be bacterial growth, but there is none present. This could be a possible error in not transferring a big enough colony of E. coli. The plate labeled ‘+pGLO, LB, ARA’ should have more distinct colonies glowing however on the UV Light figure only a film is seen glowing. This could be a cause of putting too much pressure on the colony when spreading the E. coli on the plate. Having the ability to genetically modify DNA could help researchers in the medicine field develop cells that would be resistant to any harmful thing, like the flu or HIV. In conclusion, altering bacteria’s DNA is possible and so easy it can be performed by freshmans in college.

Thursday, February 13, 2020

Accounting Essay Example | Topics and Well Written Essays - 500 words - 36

Accounting - Essay Example Failing to provide the cash flow statement, the company will lack the proper information on telling the sources of cash and its use. Additionally, the company will not have adequate planning tool for its business success in the long term (â€Å"Cash flow statements†, 2000). To improve operations, I would compare the expenses that accumulate for a given operation to its budget and provide management warning if the expenses appear to be ahead of projections (Lawrence, 2004). This will give the company time to control cost over the remaining part of the project. I would also approach the client about the increase in billing to cover the cost overrun. Assuming the costs are reimbursed to the clients, I would carry out cost precision where the customer will pay all the expenses incurred plus the revenue. Some of the accounting activities that I would take into consideration will be the cost of materials, cost of labor, and overhead. I would use cost information history to arrive at standard rates and then assign this standard costs to jobs based on the activity units (Drury, 2002). I will allocate the manufacturing overhead cost via Departmental machine hours (Innes & Mitchell, 2003). This is because the manufacturing companies studies and controls the time for direct labour and motion. They have started using machines to replace the direct labor. Using machines increases the factory overhead due to machinery depreciation, machinery maintenance, and set up of machines. Reducing the direct labor and increasing the manufacturing overhead, the correlation between the manufacturing overhead and direct labour tends to wane. Therefore, the logical response is to allocate manufacturing overhead based on the machine hours rather than direct labor hours (Carroll, 2004). I would use break-even analysis to determine the optimum output level below which will

Saturday, February 1, 2020

British Airways and its predecessor companies Essay

British Airways and its predecessor companies - Essay Example Two other airlines, Handley page, and Instone, were established using modified bombers. The three companies underwent a period of great difficulty, especially competition from French airlines, which were cheaper. To solve these problems, they merged to be joined later by British Marine Air Navigation, forming Imperial Airways. Imperial Airways began local and overseas flights immediately, flying as far as Egypt and India with a crew of 250 and a fleet of 18 crafts (Gaskell, 2010). This paper is an essay on British Airways. Later, Imperial Airways was a Brisbane, Australia route, whose duration would take grueling 12 days. The new airline added new planes such as the short S.23 C-class model, which signified that the airline was growing, as was a new carrier British Airways limited (Gaskell, 2010). After the start of the 1st World War, these two merged to form British Overseas Airways Corporation, which re-started its transatlantic flights after the war ended. In addition, they create d the BEA, a new airline to handle the European flights. At this point, the carriers needed to order new and more efficient aircraft. BOAC consequently ordered the Boeing Strato-cruiser, the Lockheed Constellation, and a Rolls-Royce engine equipped version of the DC-4. It did not take long before they ordered a jet plane, the De Havilland Comet, which dramatically reduced the length of trans-Atlantic flights (Marriott, 2010). The early 60’s saw BOAC order the Rolls-Royce Conway engine driven 707-436 to tide over until the VC-10s were ready. By 1970, with the first 747 and rapid growth, BOAC and BAL were ready to merge and work as one, establishing British Airways in 1976. BA’s most crucial year was 1976; it had a partnership with Concorde, coupled with big fleets of Lockheed TriStar and Boeing 747.

Friday, January 24, 2020

The Fall of Man :: essays research papers

Alienation: The Fall of Man through the Breaking of Moral Law Is it possible to attain or remain in a state of true happiness when you break a moral law? To many of us, road signs have been handed down through the generations and are posted clearly as the 10 Commandments delivered to us through Moses. These commandments are generally viewed as religious moral laws, but can they be viewed also as natural laws of morality for all to heed regardless of religious belief? Consider the chaos if we inverted their intended meaning, remove the word ‘not’, and apply ‘Thou Shall Kill’ or ‘Thou Shall Commit Adultery’, as new commandments. Would these new commandments conflict with natural laws of morality? The story by Andre Dubus, â€Å"Killings†, and Anton Chekhov, â€Å"The Lady with the Pet Dog†, show alienation as a horrible consequence we can suffer when a moral law is broken. In the story, â€Å"Killings†, Dubus reveals in detail, the alienation Matt and Ruth experience after Matt executes the revenge murder. In Chekhov’s story of â€Å"The Lady with the Pet Dog†, Anna and her lover, Dmitri, become familiar with alienation after they begin their extra-marital affair. The stories are completely different, however, the authors carefully guide us on a journey into brokenness and deep sadness, only to witness a deeper tragedy of self-imposed imprisonment: Alienation. Alienation caused by grave wrongdoing. Dubus and Chekhov give us insight to the effects of breaking a moral law by directly showing us how we can be impacted when we commit murder or commit adultery. Breaking moral law is shown to alienate us in ways we may not straightforwardly anticipate. In both stories, the protagonists are alienated from any notion of a supreme being. This is shown extensively through setting, tone, and action showing how the protagonist hide from their surroundings, especially from the events that are occurring or are about to occur. In â€Å"Killings†, Matt’s alienation from a supreme being begins within him at the time he begins to carry a gun, a gun that he must conceal, else face possible prison time. Matt’s friend Willis points this out when he asks, â€Å"You got a permit?†, when Matt responds that he doesn’t, then Willis seems to warn Matt, â€Å"†¦ you could get a year for that† (85), directly pointing out to him he’s breaking a law. Matt knows that for, â€Å"He had always been a fearful father† (88), and even though he still demonstrates a respect for nature and a love for his family, he now behaves indifferently about the potential consequences.

Thursday, January 16, 2020

Computerized Teachers Evaluation System

With the long history of Chinese civilization and education, formal teacher practice is quite new because for century’s teachers’ authority and power over their students cannot be challenged. Historically, learning was the major concern of education, but during the past decades, teaching quality has become an added concern of education. There are three reasons for evaluating teachers: to diagnose and help teacher improve teaching, to help students get better teaching quality and to provide a criteria for research on teaching itself. Efforts have been made by government and educators to improve teaching quality in theory and practice. Verities of book have introduced foreign evaluation theories and many articles have contributed to the theoretical and practical improvement of Chinese teacher evaluation system. However, there is no systematic record of the development of teacher evaluation process. Therefore, exploration into the development of teacher evaluation, its reform and problem become necessary and significant. In common, teachers are evaluated by students and administrators synthetically. The students’ evaluations are used as formation to help teachers improve their classroom training and activities, in–service students complete these evaluations at the end of their program, while pre-service students complete these evaluations each semester. With both single-choice and open-end questions, these evaluations are similar to all colleges. These evaluations by students are analyzed, and teachers are given a copy of the analysis, if several students make similar complaints about a teacher or about the way of course in taught, the teacher in question gets immediate feedback from his or her supervisor. The administrative evaluations are completed by the supervisor who is the training manager. These evaluations are done every semester. Statement of the Problem This study of the proposed â€Å"Computerized Teachers Evaluation System† (CTES) covers method, procedures and problems which correlate to the teacher’s evaluation. The main problem of this study is how to design, develop and implement a Computerized Teachers Evaluation System. The current system is not efficient to ensure its validity because these CTES are done manually. Specifically this study aims to answer the following common problems encountered during and after the evaluation: 1. Is there a need for computerized teachers evaluation system? 2. What are the common problems encountered during and after the evaluation? 3. What are the users expectation regarding computerized teachers evaluation? 4. What are the advantages and disadvantages of using computerized teachers evaluation system?

Tuesday, January 7, 2020

Same Sex Marriage Defies the Laws of Christianity Essay

Same Sex Marriage Defies the Laws of Christianity Today in society there are many pressures put upon people by others and their beliefs. One of the major pressures it puts on people is the pressure of homosexuality and whether it is right or wrong. Homosexuality is on the rise in the United States as well as many other countries around the world. Along with the rise of homosexuality is the rise of marriages that are taking place in these socially incorrect relationships. Throughout the country and in other countries there has been a numerous increase in the marriages of homosexuals. Same sex marriages defy the laws of Christianity, and it is immoral in the eyes of society. Homosexuality in all countries has been looked down upon†¦show more content†¦Heterosexual marriage has also been known as the best environment for the upbringing of children. Studies show that most children who are brought up in a household with a mother and a father are more productive in school and their social life. The environment which offers children the best opportunity to achieve optimal development is one comprised of their biological mother and father or a married male and female. It is in the childs best interest that this environment be fostered and protected. Children do best when they have the personal involvement and material support of a father and a mother and when both parents fulfill their responsibility to be loving providers. Studies also show that children brought up in homosexual households have the tendency to have more problems in school, work, and finding friends who dont look at them differently. Children have a fundamental liberty interest in receiving the care, companionship and society of both of their natural parents. At birth, a child has no material possessions and no hope of independent survival. 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